The In Vitro Antifungal Impact of Secondary Metabolites from Trichoderma sp. on Fusarium sp.
DOI:
https://doi.org/10.30737/agrinika.v7i2.4511Keywords:
Biocontrol agent, Fusarium sp., Secondary metabolites, Trichoderma sp.Abstract
Fusarium sp. is a significant pathogenic fungus responsible for causing wilt in various plants, including chilies, eggplants, and shallots. One approach to address this issue involves utilizing secondary metabolites from the fungus Trichoderma sp. These metabolites were applied at concentrations of 10%, 20%, and 30% alongside a fungicide called M-Dithane 45, following the recommended dosage specified on the packaging. This research aims to evaluate the impact of these secondary metabolites in suppressing the Fusarium sp. pathogen. The study occurred at the Plant Health Laboratory I within the Faculty of Agriculture at Universitas Pembangunan Nasional “Veteran” East Java from October to December 2022. It adopted a single-factor, Completely Randomized Design (CRD), and data analysis was carried out using R-studio software. In vitro analysis was conducted through the dual culture technique on a PDA medium, assessing inhibition percentages and observing the morphology of Fusarium sp. fungi. The results demonstrated that the highest inhibition percentage of Fusarium sp. occurred at a 30% concentration, reaching 26% compared to the control group. Morphological examinations of Fusarium sp. indicated that all treatments affected the growth of abnormal hyphae, including bending, curling, coiling, and lysis.
References
Agustina, D., Triasih, U., Dwiastuti, M. E., & Wicaksono, R. C. (2019). Potensi Jamur Antagonis Dalam Menghambat Pertumbuhan Jamur Botryodiplodia theobromae Penyebab Penyakit Busuk Batang Pada Tanaman Jeruk. Jurnal Agronida.
Ajayasree, T. S., & Borkar, S. G. (2018). Crop Host Specificity Of Crop Host Specificity Of Xanthomonas C Anthomonas C Anthomonas Campestris Ampestris Ampestris Pv. Vesicatoria Prevalent In Nashik District In India Alent In Nashik District In India.
Asril, M. (2011). Kemampuan Bakteri Tanah dalam Menghambat Pertumbuhan Ganoderma Boninense dan Fusarium Oxysporum Secara In Vitro dan Uji Penghambatan Penyakit Layu Fusarium pada Benih Cabai Merah. Universitas Sumatera Utara.
Contreras-Cornejo, H. A., Macías-Rodríguez, L., Del-Val, E. K., & Larsen, J. (2016). Ecological functions of Trichoderma spp. and their secondary metabolites in the rhizosphere: interactions with plants. FEMS Microbiology Ecology, 92(4), fiw036.
Djaenuddin, N. (2011). Bioekologi Penyakit Layu Fusarium Fusarium oxysporum. Seminar Dan Pertemuan Tahunan XXI PEI, PFI Komda Sulawesi Selatan Dan Dinas Perkebunan Pemerintah Provinsi Sulawesi Selatan Tanggal, 7.
Fadiji, A. E., & Babalola, O. O. (2020). Elucidating Mechanisms of Endophytes Used in Plant Protection and Other Bioactivities With Multifunctional Prospects. In Frontiers in Bioengineering and Biotechnology. https://doi.org/10.3389/fbioe.2020.00467
Laila, N., Umi, K. Y., Shafiquzzaman, S., Jannatul, F., & M, A. R. (2012). Effect of media on growth and antagonistic activity of selected Trichoderma strains against Ganoderma. African Journal of Microbiology Research. https://doi.org/10.5897/ajmr12.1216
Lestari, A. D. L. (2019). Identifikasi Jamur Pada Roti Yang Dijual Di Kota Langsa Berdasarkan Lama Penyimpanan. Jurnal Jeumpa, 6(2), 245–256.
Mukherjee, P. K., Horwitz, B. A., & Kenerley, C. M. (2012). Secondary metabolism in Trichoderma - A genomic perspective. In Microbiology. https://doi.org/10.1099/mic.0.053629-0
Purwantisari, S., & Hastuti, R. B. (2009). Isolasi dan Identifikasi Jamur Indigenous Rhizosfer Tanaman Kentang Organik di Desa Pakis, Magelang. Jurnal Bioma.
Putro, N. S., Aini, L. Q., & Abadi, A. L. (2014). Pengujian konsorsium mikroba antagonis untuk mengendalikan penyakit antraknosa pada cabai merah besar (Capsicum annuum L.). Jurnal HPT (Hama Penyakit Tumbuhan), 2(4), 44–53.
Rabha, A. J., Naglot, A., Sharma, G. D., Gogoi, H. K., & Veer, V. (2014). In vitro evaluation of antagonism of endophytic Colletotrichum gloeosporioides against potent fungal pathogens of Camellia sinensis. Indian Journal of Microbiology, 54, 302–309.
Ratnasari, J. K., & Isnawati, R. E. (2014). Uji antagonis cendawan agens hayati terhadap cendawan Cercospora musae penyebab penyakit sigatoka secara in vitro. Lenterabio, 3(2), 129–135.
Sastrahidayat, I. R. (2013). Penyakit tanaman sayur-sayuran. Universitas Brawijaya Press. Saxena, A., Raghuwanshi, R., & Singh, H. B. (2015). Trichoderma species mediated differential tolerance against biotic stress of phytopathogens in Cicer arietinum L.
Journal of Basic Microbiology, 55(2), 195–206.
Semangun, H. (1989). Penyakit-penyakit tanaman hortikultura di Indonesia. Gadjah Mada University Press.
Sholihah, R. I., Sritamin, M., & Wijaya, I. N. (2019). Identifikasi Jamur Fusarium solani yang Berasosiasi dengan Penyakit Busuk Batang pada Tanaman Buah Naga (Hylocereus sp.) Di Kecamatan Bangorejo, Kabupaten Banyuwangi. Agroekoteknologi Trop., 8, 91–
Sriyanti, N. L. G., Suprapta, D. N., & Suada, I. K. (2015). Uji keefektifan rizobakteri dalam menghambat pertumbuhan jamur Colletotrichum spp. penyebab Antraknosa pada cabai merah (Capsicum annuum L.). E-Jurnal Agroekoteknologi Tropika, 4(1), 53–65.
Sunarwati, D., & Yoza, R. (2010). Kemampuan Trichoderma dan Penicillium dalam menghambat pertumbuhan cendawan penyebab penyakit busuk akar durian (Phytophthora palmivora) secara in vitro. Seminar Nasional Program Dan Strategi Pengembangan Buah Nusantara.
Syaifudin, A. (2020). Karakterisasi Morfologis Cendawan Patogen Penyebab Layu Fusarium Pada Tanaman Cabai (Capsicum annuum L). Prosiding Seminar Nasional Indonesian R Summit, 1(1).
Vinale, F., Sivasithamparam, K., Ghisalberti, E. L., Woo, S. L., Nigro, M., Marra, R., Lombardi, N., Pascale, A., Ruocco, M., Lanzuise, S., Manganiello, G., & Lorito, M. (2014). Trichoderma Secondary Metabolites Active on Plants and Fungal Pathogens. The Open Mycology Journal. https://doi.org/10.2174/1874437001408010127
Wijaya, I., Pertanian, F., & Muhammadiyah, U. (2012). Pembiakan massal jamur Trichoderma sp. pada beberapa media tumbuh sebagai agen hayati pengendalian penyakit tanaman. Agritop Jurnal Ilmu-Ilmu Pertanian.
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